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Combined Research Techniques

Integrate study techniques and tools to unlock new opportunities in your research 



DSI solutions are used in combination with many other technologies for various research applications in both small and large animal models. The benefits of holistic research includes a greater chance of study approval and funding, a decrease in the number of animals used, the ability to identify physiological changes from multiple organ systems, and overall better science.

Synchronize implantable telemetry with hardwired data by using DSI's products listed below.
Implantable Telemetry
Signal Conditioners and Amplifiers
Signal Interface
 

Below are examples of combined techniques researchers have used. If you would like to discuss your application or learn about additional combination studies, click the schedule a free call button below. 


 




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METABOLISM AND TELEMETRY

Indirect calorimetry is the estimation of metabolic heat production by an organism from measurement of indices such as oxygen consumption or carbon dioxide production. For the purpose of most energy-expenditure studies, indirect calorimetry measures the volume of expired air per unit of time and determines the percentage of oxygen utilized. These data are then used for estimating the metabolic rate (energy expenditure) and respiratory exchange ratio (relative measurement of fat, carbohydrate and protein oxidation). Indirect calorimetry systems contain air tight chambers, so measuring physiological endpoints during indirect calorimetry testing is difficult. Our Panlab Oxylet Pro systems have all been tested and validated to properly work in combination with DSI implantable telemetry, allowing for the acquisition of meaningful and high impact holistic in vivo data. 

Common endpoints achieved through the use of telemetry include ECG, blood pressure, continuous blood glucose, temperature, activity, and more. 


Picture1
Telemetry with Indirect Calorimetry, Intake
and Activity

Treadmill_RPC1V1
Telemetry with Indirect Calorimetry
Treadmill


Oxylet Waste Collection
Telemetry with Indirect Calorimetry 
and Waste Collection 

COMBINATION BENEFITS
  • Real-time cardiovascular, temperature, glucose, and respiratory metabolism data
  • A better understanding of clusters of conditions and related activity that are often associated with metabolic diseases, cardiovascular disease, and diabetes
  • Decreased handling in home cage environments, while obtaining high quality physiological data, that minimizes animal stress and increased translation
  • A more comprehensive, holistic picture of whole animal physiology
  • Comprehensive approach to understanding the animal body mass and physiological phenotype


FUNCTIONS AND PARAMETERS
  • Heart Rate, Blood Pressure, ECG
  • Continuous Glucose Monitoring
  • EEG, EMG, Sleep
  • Respiratory Rates
  • Body Temperature
  • VO2 Consumption, VCO2 Production, Respiratory Exchange Ratio and Energy Expenditure
  • Food and Drink Consumption
  • Urine and feces collection
  • Activity


APPLICATIONS



WHOLE BODY PLETHYSMOGRAPHY AND TELEMETRY


Whole body plethysmography (WBP) and telemetry remain the recommended techniques for obtaining physiological assessments in small animals. Combining respiratory WBP with cardiovascular or CNS telemetry endpoints provides researchers looking at holistic effects and multi organ interactions with powerful data from one subject. 




COMBINATION BENEFITS
  • Obtain both respiratory and cardiovascular endpoints
  • Longitudinal study approaches
  • Understand the interactions between organ systems
  • Utilize the same Ponemah software platform
  
FUNCTIONS AND PARAMETERS
  • Heart Rate, Blood Pressure, ECG
  • EEG, EMG, Sleep
  • Respiratory Rates, Volumes, Flows
  • Resistance
  
APPLICATIONS
  • Sleep apnea
  • Earlier drug development safety assessments
  • Circadian Rhythm
  • Cardiorespiratory interactions 
  • Heart failure
  • Spinal injury
  • Anxiety
  • Recreational and addictive drug effects
  • Epilepsy  
Selected References Combining WBP with Telemetry:

The role of GαO‐mediated signaling in the rostral ventrolateral medulla oblongata in cardiovascular reflexes and control of cardiac ventricular excitability
Effects of Inhaled Aerosolized Carfentanil and Subsequent Naloxone Treatment on Real-Time Physiological Responses in Mice
Effects of inhaled aerosolized carfentanil on real-time physiological responses in mice: a preliminary evaluation of naloxone

 
 
Opioid addiction webinar highlights combined use of EEG telemetry and plethysmography
 
 
 


WHOLE BODY PLETHYSMOGRAPHY AND TETHERED SYSTEMS

Specially designed respiratory whole body plethysmographs (WBP) offer the flexibility to integrate tethered animal models; allowing the ability to combine technologies or have access to the animal while in the chamber.

  • Optogenetics - Neural control of breathing
  • Electrical Commutator - Collect hardwired biopotential (EEG, EMG, EOG) signals simultaneously
  • Fluid Swivel - Administer compound, manage pain, draw blood, or create an injury model

Suitable for mouse, rat, and guinea pig animal models



WBP Tower Rat with Optogenetics and extension ring
Optogenetics

WBP Rat Tower with Swivel
Fluid Swivel


WBP Tower Rat with commutator and extension ring
Electrical Commutator

COMBINATION BENEFITS
  • Obtain additional physiological data to understand multi-organ interaction
  • Stimulate or record using tethered technology
  • Vascular access for delivery or sampling
FUNCTIONS AND PARAMETERS
  • Heart Rate, Blood Pressure, ECG, EOG
  • EEG, EMG, Sleep
  • Respiratory Rates, Volumes, Flows
  • Resistance
APPLICATIONS
  • Sleep apnea
  • Respiratory depression
  • Pain management
  • Recreational and addictive drug effects
  • Cardiorespiratory stimulation
  • Hypoxia/hypercapnia effects
  • Anxiety
  • Epilepsy/Seizures
  • Blood sampling / drug administration