Buxco Small Animal Whole Body Plethysmography

Mouse 4 site WBP 017
FP_WBP_software
WBP is an acronym for Whole Body Plethysmography.  WBP is a way to measure respiratory function in animals (or people) without the use of anesthesia or restraint.  The system relies on a specially designed chamber in which the subject is placed.  A sensitive pressure transducer is connected to measure pressure changes inside the chamber.  Since the chamber is fitted with screen pneumotachs which allow air to pass outside the chamber, the small pressure changes in the chamber are related to flow.  And so, the chamber is a plethysmograph.  From these small flows signals, utilizing special algorithms Buxco FinePointe software derives ventilatory and bronchio constriction parameters.

Key Features:

  • Species:  Mouse, Mouse Pup (P3 and older), Rat, GP and other species up to 1kg.
    For larger animals refer to Large Animal WBP page

  • Up to 16 animals configured using 2 site or 4 site hardware controllers

  • Integrated, automated, software regulated bias flow

  • Pre-calibrated digital temperature and humidity

  • Integrated nebulizer controller per channel for simultaneous exposure

  • Integrated 4-port gas automation

  • Detachable control console for equipment setup flexibility (4-Site only)

  • Automated calibration for up to 4 chambers with a single click

  • Automated microprocessor-controlled system diagnostics

  • Combination Studies - option to integrate fluid swivels, electrical commutators, and optogenetics (LED)

 

 

 

Mouse Pup WBP Open
Mouse pup whole body plethysmograph

Key Benefits

  • Auto diagnostics – microprocessor-controlled system check ensures data accuracy

  • Smart integration – bidirectional communication ensures scientific reproducibility

  • Auto calibration – reduces user error and provides consistent, fast calibration

  • Halcyon – chambers incorporate patented noise suppression patented design

  • Nebulizer calibration – overcomes variability in nebulizer efficiency rates

  • Automated bias flow rates – user defined bias flow reduction during exposure period

  • Ultra low bias flow noise – minimizes signal to noise ratio, critical for WBP analysis

  • Finepointe software –integrated study design and subsequent automated reporting

  • Gas Automation – control delivery of up to 4 different gases into chambers using bias flow lines

  • State of the art acrylic manufacturing techniques yield the highest quality chambers on the market

WBP Derived Parameters

  • View List of WBP Derived Parameters

Apnea-Specific Derived Parameters

  • View List of Apnea Derived Parameters

Photo Gallery

Applications & References

Publications citing FinePointe whole body plethysmography

Bosnjak B, Tilp C, Tomsic C, Dekan G, Pieper M, Erb K, EpsteinM. “Tiotropium bromide inhibits relapsing allergic asthma in BALB/c mice.” Pulmonary Pharmacology and Therapeutics. 2014;27:44-51.

Glynn A, Alves D, Frick O, Erwin-Cohen R, Porter A, Norris S, Waag D, Nalca A. “Comparison of experimental respiratory tularemia in threenonhuman primate species.” Comparative Immunology, Microbiology and Infectious Diseases. 2015;39:13-24

Haapakoski R, Karisola P, Fyhrquist N, Savinko T, Wolff H, Turjanmaa K, Palosuo T, Reunala T, Lauerma A, Alenius H. “Intradermal cytosine-phosphate-guanosine treatment reduces lung inflammation but induces IFN-gamma-mediated airway hyperreactivity in a murine model of natural rubber latex allergy.” American journal of respiratory cellular and molecular biology. 2011;44:639-647.

Rajavelu P, Chen G, Xu Y, Kitzmiller J, Korfhagen T, Whitsett J. “Airway epithelial SPDEF integrates goblet cell differentiation and pulmonary Th2 inflammation.” Journal of Clinical Investigation. 2015.

Viby N, Isidor M, Buggeskov K, Poulsen S, Hansen J, Kissow K. “Glucagon-Like Peptide-1 (GLP-1) Reduces Mortality and Improves Lung Function in a Model of Experimental Obstructive Lung Disease in Female Mice.” Endocrinology. 2013;154:4503–4511.

Accessories

A variety of accessories and supplies are available for all Buxco respiratory products to improve system flexibility and maintain proper system performance.  The most commonly requested accessory is the Chamber Maintenance Kit which includes replacement o-ring seals and plethysmograph screens.

1650
Whole body plethysmography articles citing DSI in Google Scholar


Available Chambers (all types)

Small Animal WBP - Key Features
Invasive Non-Invasive
Restrained Un-Restrained*
Acute Longitudinal
Small Animal Large Animal
End Points
Rate / Volumes / Flows
Resistance
Compliance
Lung Characterization
Integrated Drug Delivery Methods
Nebulizer
IV
Tethered Infusion
Common Applications
Asthma
Model Development
Treatment Assessment
Apnea & Breath Scoring
Sleep Apnea
Central Apnea
Sniffs
COPD
Emphysema
Chronic Bronchitis
Lung Fibrosis
Pulmonary Fibrosis
Cystic Fibrosis
Safety Assessment
Safety Pharmacology
Toxicology
Acute Respiratory Disorders
Respiratory Depression
Respiratory Syncytial Virus (RSV)
Acute Respiratory Distress Syndrome (ARDS)
Mucociliary Clearance and Dysfunction
Pneumonia
Cough
Tuberculosis (TB)
Bronchiolitis/ Bronchitis