FinePointe for Whole Body Plethysmography

Mouse 4 site WBP 017
FP_WBP_software

WBP is an acronym for Whole Body Plethysmography.  WBP is a way to measure respiratory function in animals (or people) without the use of anesthesia or restraint.  The system relies on a specially designed chamber in which the subject is placed.  A sensitive pressure transducer is connected to measure pressure changes inside the chamber.  Since the chamber is fitted with screen pneumotachs which allow air to pass outside the chamber, the small pressure changes in the chamber are related to flow.  And so, the chamber is a plethysmograph.  From these small flows signals, utilizing special algorithms Buxco FinePointe software derives ventilatory and bronchio constriction parameters.

Key Features:

  • Species:  Mouse, Mouse Pup, Rat, GP and other species up to 1kg.
    For larger animals refer to Large Animal WBP data sheet

  • Up to 16 animals configures in 2 or 4 site controllers.

  • Integrated, automated, software regulated bias flow

  • Pre-calibrated digital Temperature and Humidity

  • Integrated nebulizer controller

  • Integrated gas automation

  • Detachable control console for equipment setup flexibility (4-Site only)

  • Automated calibration for up to 4 chambers with a single click

  • Automated system microprocessor diagnostics during calibration process

  • Combination Studies - integrate fluid swivels or electrical commutators 

Key Benefits

  • Auto diagnostics – microprocessor controlled system check ensures accurate data

  • Smart Integration – bidirectional communication ensures scientific reproducibility

  • Auto Calibration – reduces user error and provides consistent, fast calibration

  • Halcyon – Chambers incorporate patented noise suppression patented design

  • Nebulizer calibration – overcomes variability in nebulizer efficiency rates

  • Automated flow rate reduction during exposure

  • Low BIAS flow noise –minimize signal to noise ratio, critical in WBP

  • Finepointe Software –integrated study design and subsequent automated reporting

  • Gas Automation – Up to 4 different gasses can be switched by software, and integrated through controller into chambers

Derived Parameters

Parameter

Description

Units

f

Respiratory Rate

BPM

TVb

Tidal volume (volume inhaled) estimated from the Box Flow signal. It is equal to the product of the volume measured from the Box Flow and Comp

mL

MVb

Minute volume (the rate of ventilation) estimated from the Box Flow signal. It is equal to the product of the volume measured from the Box Flow, f, and Comp

mL/min

Penh

Index of constriction.

None

PAU

Index of constriction.

None

Rpef

The location into expiration where the peak occurs as a fraction of Te

None

Comp

The factor applied to box flow to estimate animal’s flow

None

PIFb

Estimated peak inspiratory flow

mL/s

PEFb

Estimated peak expiratory flow

mL/s

Ti

Inspiratory time

S

Te

Expiratory time

S

EF50

Expiratory flow at 50% expired volume (Uncompensated)

mL/s

EIP

End inspiratory pause. Time it takes to transition from inspiration to expiration

ms

EEP

End expiratory pause. Time it takes to transition from expiration to inspiration

ms

Tr

Relaxation time.

S

TB

Duration of breaking. Percentage of the breath occupied by transitioning from inspiration to expiration

%

TP

Duration of pause before inspiration. Percentage of the breath occupied by transition from expiration to inspiration

%

Tbody

Body temperature. Either assumed or measured using telemetry

C

Tc

Chamber temperature. Either assumed or measured using digital temperature sensor in the chamber

C

RH

Relative humidity. Either assumed or measured using digital humidity sensor in the chamber

%

Rinx

Rejection index. Percentage of breaths rejected before a breath is accepted.

%

Applications & References

Publications citing FinePointe whole body plethysmography

Bosnjak B, Tilp C, Tomsic C, Dekan G, Pieper M, Erb K, EpsteinM. “Tiotropium bromide inhibits relapsing allergic asthma in BALB/c mice.” Pulmonary Pharmacology and Therapeutics. 2014;27:44-51.

Haapakoski R, Karisola P, Fyhrquist N, Savinko T, Wolff H, Turjanmaa K, Palosuo T, Reunala T, Lauerma A, Alenius H. “Intradermal cytosine-phosphate-guanosine treatment reduces lung inflammation but induces IFN-gamma-mediated airway hyperreactivity in a murine model of natural rubber latex allergy.” American journal of respiratory cellular and molecular biology. 2011;44:639-647.

Rajavelu P, Chen G, Xu Y, Kitzmiller J, Korfhagen T, Whitsett J. “Airway epithelial SPDEF integrates goblet cell differentiation and pulmonary Th2 inflammation.” Journal of Clinical Investigation. 2015.

Viby N, Isidor M, Buggeskov K, Poulsen S, Hansen J, Kissow K. “Glucagon-Like Peptide-1 (GLP-1) Reduces Mortality and Improves Lung Function in a Model of Experimental Obstructive Lung Disease in Female Mice.” Endocrinology. 2013;154:4503–4511.

Glynn A, Alves D, Frick O, Erwin-Cohen R, Porter A, Norris S, Waag D, Nalca A. “Comparison of experimental respiratory tularemia in threenonhuman primate species.” Comparative Immunology, Microbiology and Infectious Diseases. 2015;39:13-24

Accessories

A variety of accessories are available for DSI's line of whole body plethysmography products. The most commonly requested accessories include the following:

  • Mouse Halcyon WBP maintenance kit

  • Rat Halcyon WBP maintenance kit

  • Guinea Pig Halcyon WBP maintenance kit

1650
Whole body plethysmography articles citing DSI in Google Scholar


Available Chambers (all types)

WBP Key Features

Invasive

Non-Invasive

Restrained

Un-Restrained

Acute

Longitudinal

Small Animal

Large Animal

End Points

Rate / Volumes / Flows

Resistance

Compliance

Lung Characterization

Common Applications

Asthma

Sleep Apnea

COPD

Fibrosis

Tuberculous

Cough

Respiratory Depression

Safety Assessment

Integrated Drug Delivery Methods

Nebulizer

IV

Tethered Infusion