Buxco® FinePointe Non-Invasive Airway Mechanics

(Double Chamber Plethysmography)
NAM 2 site_fr
NAM mouse chamber_LR
NAM01

NAM is an acronym for Noninvasive Airway Mechanics also known as “Pennock” Double Chamber Plethysmography.  The NAM system monitors ventilatory and bronchoconstriction parameters in animals without the use of anesthesia.  The subject is restrained in a double chamber plethysmograph which allows the independent measurement of nasal and thoracic flows.  Thoracic flow being  the airflow due to thoracic expansion and compression.  When the subject inhales, it draws air from the nasal chamber into the lungs. As the air moves into the lungs, the chest expands and forces the same volume of air out of the thoracic chamber. The phase difference between both flows is a measure of bronchoconstriction and called specific airway resistance (sRaw).  The Double Chamber Plethysmograph design was scientifically established over 30 years ago and is considered an alternative to EF50 and Penh1.  The NAM system is ideal for researchers interested longitudinal studies of airway resistance without the use of anesthesia.

Key Features:

  • Species: Mouse, Rat, Hartley Guinea Pig
  • Continuously measures specific airway resistance in conscious animals
  • 2 and 4 chamber systems available
  • 12 chambers can be monitored simultaneously with one software license
  • Hardware controller supports chambers for all three species
  • Integrated nebulizer controller per chamber for simultaneous software controlled exposure
  • Automated microprocessor-controlled system diagnostics
  • Chambers utilize patented Allay™ Restraint system, integrated pneumotach, and reference chambers
  • Integrated, automated, software regulated “low noise” bias flow
  • Thoracic chamber bias flow can be enabled to help regulate body temperature
  • Automated calibration including calculation of Time Constants for Nasal and Thoracic portions of chamber

Key Benefits:

  • Patented Allay™ Restraint™ - secures the animal without compressing the thorax
  • Auto Diagnostics – microprocessor controlled system check ensures accurate data
  • Smart Integration – bidirectional communication ensures scientific reproducibility
  • Auto Calibration – reduces user error and provides consistent, fast calibration
  • Halcyon® Pneumotach – chambers incorporate patented noise suppression design
  • Nebulizer calibration – overcomes variability in nebulizer efficiency rates
  • Automated bias flow rates – user defined bias flow reduction during exposure period
  • Ultra-low bias flow noise – minimizes signal to noise ratio, improves data analysis
  • FinePointe Software – integrated study design and subsequent automated reporting
  • State-of-the-art acrylic manufacturing techniques - yields the highest quality chambers available

Buxco NAM Chamber Details

  • View NAM chambers

NAM Derived Parameters

  • View List or NAM Derived Parameters

Applications and References

1Pennock, B.E., C.P. Cox, R.M. Rogers, W. A. Cain, and J.H. Wells. A noninvasive technique for measurement of changes in specific airway resistance. J. Appl. 46(2): 399-406, 1979.

 

Publications Citing Buxco FinePointe Noninvasive Airway Mechanics

Khaitov M, Shilovskiy I, Nikonova A, Shershakova N, Kamyshnikov O, Babakhin A, Zverev V, Johnston S, Khaitov R. “Small interfering RNAs targeted to interleukin-4 and respiratory syncytial virus reduce airway inflammation in a mouse model of virus-induced asthma exacerbation.” Human Gene Therapy. 2014;25:642-650.

Lowe A, Broadley K, Nials A, Ford W, Kidd E. “Adjustment of sensitisation and challenge protocols restores functional and inflammatory responses to ovalbumin in guinea-pigs.” The Journal of Pharmacological and Toxicological Methods. 2014;72: 85-93.

Kandasamy R, Park S, Boyapalle S, Mohapatra S, Hellermann G, Lockey R, Mohapatra S. “Isatin down-regulates expression of atrial natriuretic peptide receptor A and inhibits airway inflammation in a mouse model of allergic asthma.” International Immunopharmacology. 2010;10:218-225.

Accessories / Supplies:

A variety of accessories and supplies are available for all Buxco respiratory products to improve system flexibility and maintain proper system performance.  The most commonly requested accessory is the Chamber Maintenance Kit which includes replacement o-ring seals and plethysmograph screens.

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Non-invasive Airway Mechanics articles citing DSI in Google Scholar

NAM - Key Features
Invasive Non-Invasive
Restrained Un-Restrained
Acute Longitudinal
Small Animal Large Animal
End Points
Rate / Volumes / Flows
Resistance
Compliance
Lung Characterization
Integrated Drug Delivery Methods
Nebulizer
IV
Tethered Infusion
Common Applications
Asthma
Model Development
Treatment Assessment
Apnea & Breath Scoring
Sleep Apnea
Central Apnea
Sniffs
COPD
Emphysema
Chronic Bronchitis
Lung Fibrosis
Pulmonary Fibrosis
Cystic Fibrosis
Safety Assessment
Safety Pharmacology
Toxicology
Acute Respiratory Disorders
Respiratory Depression
Respiratory Syncytial Virus (RSV)
Acute Respiratory Distress Syndrome (ARDS)
Mucociliary Clearance and Dysfunction
Pneumonia
Cough
Tuberculosis (TB)
Bronchiolitis/ Bronchitis