As you return to the lab and work to get your research back up and running, are there new capabilities or more in-depth tools you need? DSI has been serving cardiovascular researchers for over 35 years with several revolutionary, first-to-market solutions while putting reliable high-quality data at the center of it all. We are proud to be part of the Harvard Bioscience family of brands to bring even more value to this critical community. The following is a high-level overview of solutions spanning in vitro, ex vivo, and in vivo applications and citations for publications where they have been used.
To help you get started in the lab sooner, DSI also offers a range of services including system and software training, surgical, data analysis, and system validation. Avoid unnecessary delays and order animals pre-implanted with DSI telemetry. Have a mountain of data to analyze from studies performed before quarantine? Let our expert analysts take care of it for you and provide custom reports. Visit our website or connect with us at the upcoming AHA Hypertension and Safety Pharmacology Society virtual meetings to learn more!
Cardiovascular Research Solutions
Collect and analyze continuous physiologic data from conscious, freely moving laboratory animals. DSI’s implantable telemetry can accommodate a wide variety of animal sizes, from transgenic mice to nonhuman primates. Available endpoints include blood pressure, left ventricular pressure, electrocardiogram (ECG), pulmonary pressure, temperature, activity, and more. Telemetry is used in many areas of cardiovascular research including basic cardiovascular sciences, drug discovery, safety pharmacology, infectious disease, and toxicology.
Download our free whitepaper to learn about advanced cardiovascular biomarkers available with DSI telemetry such as heart rate variability, pulse wave velocity, baroreflex, and more!
There are over 7,000 cardiovascular publications on Google Scholar citing use of DSI technology! Visit our website to access additional publications.
Kurtz TW, Griffin KA, Bidani AK, Davisson RL, Hall JE. (2005). “Recommendations for blood pressure measurement in humans and experimental animals: part 2: blood pressure measurement in experimental animals: a statement for professionals from the Subcommittee of Professional and Public Education of the American Heart Association Council on High Blood Pressure Research”. Arteriosclerosis, Thrombosis, and Vascular Biology, 25(3):e22-33. https://doi.org/10.1161/01.atv.0000158419.98675.d7
Forced and Voluntary Exercise
Treadmills and activity wheels are popular tools for studying the physiologic adaptations and possible benefits from exercise. Panlab and Coulbourn Instruments behavioral solutions can be enhanced with the integration of telemetry for measurement of cardiovascular endpoints to capture and interpret the full cardiovascular response. Treadmills can also be combined with respiratory metabolism (V02max) evaluation for real time observation of physiologic changes and performance during exercise.
Lakin R, Guzman C, Izaddoustdar F, Polidovitch N, Goodman JM,Backx PH. (2018). “Changes in Heart Rate and Its Regulation by the Autonomic Nervous System Do Not Differ Between Forced and Voluntary Exercise in Mice”. Frontiers in Physiology, 9: 841. https://dx.doi.org/10.3389%2Ffphys.2018.00841
Harvard Apparatus syringe pumps are used in cardiovascular research involving drug discovery, tissue engineering, and microfluidic modeling. Our syringe pumps provide accurate and repeatable dosing for pharmaceutical drugs with the potential to alleviate cardiac arrythmia. Harvard Apparatus syringe pumps provide precise flow rates to allow high cell seeding yields for tissue engineering and successful lab-on-a-chip experiments for identification of cardiac markers.
We understand that sometimes your research requires infusion tools that can adapt to your study designs. The iPRECIO® Micro Infusion Pump from Primetech Corporation is the world’s first implantable, programmable, refillable infusion pump for mice and other small animals. Customize infusion of new drug compounds, fluids, and more. iPRECIO can be combined with DSI’s implantable telemetry for comprehensive analysis of physiologic effects associated with the infused substance.
Kuroki MT, Fink GD, Osborn JW. (2014). “Comparison of arterial pressure and plasma ANG II responses to three methods of subcutaneous ANG II administration”. American Journal of Physiology Heart and Circulatory Physiology, 307(5): H670–H679. https://dx.doi.org/10.1152%2Fajpheart.00922.2013
Isolated Heart Perfusion
Hugo Sachs offers isolated heart systems for cardiac physiology measurements in both Langendorff and Working Ejecting Heart perfusion models. These modular systems are tailored to your needs and can be used for hearts ranging in size from mouse to pig. This system is suitable for both GLP and non-GLP environments with the ability to combine DSI’s Ponemah software with the Hugo Sachs platform.
Jensen K, Skarsfeldt MA, Stærkind H, et. al. (2018). “Magnetocardiography on an isolated animal heart with a room-temperature optically pumped magnetometer”. Nature Scientific Reports, 8:16218. https://doi.org/10.1038/s41598-018-34535-z
Microelectrode Array Monitoring
Multi Channel Systems offers fully integrated amplifier systems to analyze parameters including beat frequency, signal propagation (direction and speed), and QT interval effects. The system can be used for in vitro and in vivo recordings including isolated/iPS derived cardiomyocytes, retrograde-perfused isolated organs in a Langendorff setup, and acute open thorax. These solutions serve basic research applications, safety pharmacology, and toxicology screening approaches.
Takasuna K, Asakura K, Seiichi Araki, et. al. (2017). “Comprehensive in vitro cardiac safety assessment using human stem cell technology: Overview of CSAHi HEART initiative”. Journal of Pharacological and Toxicological Methods, 83:42-54. https://doi.org/10.1016/j.vascn.2016.09.004
Cardiac Action Potential
Perform cardiac action potential recordings on cells, SA nodes, and ventricular slices for characterization of bioactive compounds, including the hERG channel testing for early identification of QT interval prolongation. HEKA Elektronik, Warner Instruments, and Multichannel Systems have a variety of patch clamp solutions, tailored to meet your needs.
Goversen B, Becker N, Stoelzle-Feix S, et. al. (2018). “A Hybrid Model for Safety Pharmacology on an Automated Patch Clamp Platform: Using Dynamic Clamp to Join iPSC-Derived Cardiomyocytes and Simulations of Ik1 Ion Channels in Real-Time”. Frontiers in Physiology. https://doi.org/10.3389/fphys.2017.01094
Want to see more?
Learn about all the cardiovascular solutions offered by Harvard Bioscience brands, spanning in vitro, ex vivo, and in vivo, here.
Are you looking for solutions to enhance your cardiovascular research? Visit our virtual AHA Hypertension (September 10-13th) or Safety Pharmacology Society (September 13-16th) booths. If you are not attending one of these meetings, or would like to speak to someone sooner, contact us today, and let’s put together a plan to ensure your success!
If you are interested in learning more about physiologic monitoring solutions or services from DSI, schedule a call with us when it’s convenient for you.
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