Understanding physiology and behavior requires tools that can capture dynamic biological signals in freely moving animals. While implantable telemetry and behavioral assays provide rich functional data, measuring circulating signaling molecules longitudinally has remained a major technical challenge.
In this webinar, researchers from HHMI Janelia will present a chronic jugular microdialysis workflow that enables continuous sampling of blood chemistry in freely moving mice. Attendees will learn how the method was developed—including surgical approach, probe design iterations, and workflow optimization—and how coupling microdialysis with mass spectrometry enables measurement of hundreds of circulating amines and small peptides over time.
This session will highlight how chronic jugular microdialysis can complement existing telemetry, behavioral, and vascular access approaches, enabling researchers to link molecular dynamics with physiological and behavioral state in ways not previously possible.
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Learning Objectives
By attending this webinar, participants will be able to:
• Explore how longitudinal blood chemistry measurements can reveal physiological state changes and their relationship to behavior
• Understand how chronic jugular microdialysis enables longitudinal measurement of circulating molecules in freely moving animals
• Learn practical considerations for implementing jugular microdialysis, including surgical approach, probe selection, and workflow optimization
• Identify how blood microdialysis can complement implantable telemetry, behavioral monitoring, and other in vivo techniques
• Gain insight into probe development and technical challenges associated with chronic vascular microdialysis
• Evaluate how chronic jugular microdialysis could be applied to their own research in neuroscience, metabolism, pharmacology, or systems physiology