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Workshop: Animal Handling Techniques for Respiratory Research Applications in Rodents


This workshop is sponsored by Data Sciences International and will take place during the National AALAS meeting.

A variety of techniques and equipment are available to study the pulmonary/respiratory system. During this workshop, focus will be placed on animal handling and techniques for the following research applications: anesthetized resistance and compliance (RC), improved restraint techniques for conscious rodent applications, and free-roaming whole body plethysmography (WBP) for rodents. RC systems directly measure the animal’s respiratory flow and airway pressure in anesthetized animals. A tracheostomy will be demonstrated by an expert small animal surgeon and then attendees will perform a tracheostomy under the guidance of an experienced surgeon. Discovery, Safety Pharmacology and Toxicology researchers use conscious restrained animals in various applications, including inhalation/exposure and head-out chambers for collection of respiratory data. Benefits of newer restraint technology over the traditional plunger-style restraint will be presented, including reduced loading and acclimation time while providing maximum subject comfort. WBP systems monitor the respiration of conscious, unrestrained animals for high throughput screening or chronic data collection sessions. The benefits of the WBP application and data will be presented and attendees will learn how to successfully calibrate the plethysmography system, as well as how to place an animal in a chamber. Attendees will have the opportunity to place animals using both restraint methods. Comfort with rodent handling is strongly recommended for participation.

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About DSI Events

Data Sciences International (DSI) is involved in over 60 events every year. These include scientific meetings such as industry trade shows, courses and seminars, workshops, as well as educational user groups and symposia all over the world.

DSI supported educational events are scientific meetings organized in cooperation with a local institution or society, pharmaceutical company, university, or local telemetry user group. Educational event meetings serve as an educational forum in which researchers can freely share current scientific information with their peers, students, and other interested scientists.

Common to the general theme of the meetings is the data collected through physiological monitoring of freely moving conscious laboratory animals made possible through the use of fully implantable telemetry technology.

Presentations and posters on the latest methods employed by the presenting researchers enable those attending to benefit from the as yet unpublished work of peers. Presentations can be on a wide range of related research topics including surgical implantation methods, data analysis methods, treatment effects of new pharmaceutical compounds, new animal models, behavioral and physiological interactions, basic research on physiological systems, and numerous other whole animal chronic monitoring research topics.

Meetings are held in classroom style with ample time allowed for questions and discussion among the participants. Typical audience sizes run from 40 to 120.